منابع مشابه
High throughput pH optimization of protein crystallization.
Most high throughput structural proteomics centers use the sitting-drop method to obtain diffracting crystals for three-dimensional (3D) structure determination of biological macromolecules by x-ray crystallography. Although several robotic systems are available for dispensing the initial sitting-drop screening conditions, generally they are not used for optimization of crystallization conditio...
متن کاملHigh-throughput protein purification and quality assessment for crystallization.
The ultimate goal of structural biology is to understand the structural basis of proteins in cellular processes. In structural biology, the most critical issue is the availability of high-quality samples. "Structural biology-grade" proteins must be generated in the quantity and quality suitable for structure determination using X-ray crystallography or nuclear magnetic resonance (NMR) spectrosc...
متن کاملLaboratory Automation and High- Throughput Chemistry High-Throughput Protein Purification and Quality Assessment for Crystallization
The ultimate goal of structural biology is to understand the structural basis of proteins in cellular processes. In structural biology, the most critical issue is the availability of high-quality samples. Structural biology-grade proteins must be generated in the quantity and quality suitable for structure determination using x-ray crystallography or nuclear magnetic resonance (NMR) spectroscop...
متن کاملPolymorph Characterization using Raman Spectroscopy in High Throughput Crystallization Studies
Solid dose drugs make up a considerable part of the pharmaceutical market. A solid dose form of a drug can offer long shelf life, convenient ways of drug delivery, and can be packaged into a variety of forms such as capsules, tablets, gel caps, and patches. Polymorphism is the ability of a molecule to exist in more than one stable or meta-stable crystalline state. The distinct crystalline forms...
متن کاملThe open-access high-throughput crystallization facility at EMBL Hamburg.
Here, the establishment of Europe's largest high-throughput crystallization facility with open access to the general user community is reported. The facility covers every step in the crystallization process from the preparation of crystallization cocktails for initial or customized screens to the setup of hanging-drop vapour-diffusion experiments and their automatic imaging. In its first year o...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 2005
ISSN: 0108-7673
DOI: 10.1107/s0108767305093633